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Fura-2

 

Fura-2

Fura-2 is a ratiometric fluorescent dye which binds to free intracellular calcium. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly correlated to the amount of intracellular calcium. The use of the ratio allows freedom from a myriad of confounding factors, such as ambient light, making Fura-2 one of the most preferred tools to quantify calcium levels.



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